![]() ![]() This multiomic approach characterizes direct linkages between gene expression and regulatory elements across tens of thousands of cells giving a more comprehensive view of the cell state, differential expression drivers and functional differences within cell populations. With as little as a few hundred cells, scATAC-Seq allows profiling of chromatin structure at the single cell level, providing an unprecedented view of the heterogeneity of chromatin regulation.Ĭhromium Single Cell Multiome + Gene Expression unites transcriptional and chromatin landscapes from a single nucleus. The 10x Genomics’ Chromium Single Cell ATAC Sequencing Solution (scATAC-Seq) is capable of identifying rare and important cellular subtypes that cannot be acquired in amounts sufficient for genome-wide chromatin analyses. Assay for Transposase-Accessible Chromatin (ATAC-seq) coupled with next-generation sequencing is a technique to identify accessible chromatin regions. These chromatin alterations within the genome can reveal a great deal about the state of the cell. The tightly packed eukaryotic genome relies on the highly regulated alteration of chromatin structure to facilitate the accessibility of genomic regions for gene expression. Flex allows for the targeting of as few as 500 cells up to 128k cells with multiplexing and pooling. The assay is a probe-based chemistry that requires less sequencing depth than traditional scRNAseq, and covers 18,000 human genes or 19,000 mouse genes, including mitochondrial genes for assessing sample quality. Flex provides flexibility in the sample preparation process and experimental design by enabling batching across time and collection sites, cell surface protein labelling, sample multiplexing with unique sample barcodes, infectious agent neutralization, and the potential for cost savings. The Flex assay allows for use of precious samples that were previously inaccessible due to logistical challenges in sample handling. As of September 2023, we have run over 1500 scRNAseq samples from over 100 investigators across 9 different species, giving us confidence in the data we generate.”ġ0x Genomics’ Flex Assay: Gene expression profiling of single cell/nuclei samples fixed with formaldehyde or isolated from FFPE tissue blocks. The Molecular Genomics Core has been carrying out scRNAseq since 2016. Given the ability of the technique to identify subtle expression changes at a single cell level, the approach is ideal for developing normative expression in atlas-type studies or assessing the impact that disease or treatment states may have on tissues and cells. From translational immunology and immuno-oncology, to infectious disease research-these technological advancements, along with our intuitive software analysis and visualization tools, will accelerate the understanding of the adaptive immune system.Single cell transcription profiling provides the opportunity to stratify complex cell types from heterogeneous tissue, to identify rare cell types, and to characterize subpopulations from seemingly homogeneous populations of cells.A comprehensive approach to simultaneously examine the cellular context of the adaptive immune response and the immune repertoires of hundreds to millions of T and B cells on a cell-by-cell basis.The Chromium Single cell Immune Profiling Solution ![]() Encapsulate up to 80,000 cells in less than 10 minutes.More efficient than leading academic droplet systems.Complete practical solution for single cell analysis Identify rare cell types in heterogeneous populations.High-throughput single cell transcriptomic measurements enable discovery of gene expression dynamics for profiling individual cell types.Key advantages of the Chromium Single cell RNA-Seq Finally, the Chromium™ Software Suite is utilized for processing, analysis and visualization of single cell gene expression data. High-quality next generation sequencing libraries are finished in single bulk reaction. Lysis and barcoded reverse transcription of polyadenylated mRNA from single cells are performed inside each GEM. Single cells, reagents and a single Gel Bead containing barcoded oligonucleotides are encapsulated into nanoliter-sized GEMs (Gel Bead in emulsion) using the GemCode Technology. The Chromium™ Single Cell RNA sequencing Solution The GemCode™ Technology from 10X Genomics, which combines microfluidics with molecular barcoding and custom bioinformatics software enable analyzing the transcriptomes of thousands of cells at single cell resolution. Advances in single cell RNA quantification techniques have enabled comprehensive study of cell subpopulations within a heterogeneous population.
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